JET LAG AND CIRCADIAN CLOCK

REPORT

 

Prepared by:

Francisco J. Collazo Beauchamp

 

 

 

 

 

 

 

 

 

 

 

 

Edited by:  Billie Foster

 

February 19, 2003


 

ABSTRACT

 

 

The purpose of the report is to outline the disturbances of sleep by the circadian rhythm, the master clock of the body.  The report is comprised of several articles published by medical professionals in the field of sleep disorders.  The time zones influence the interaction between the sleep cycle and hormone production activity. Certain hormones peak in the evening and reach a low level in the morning; others have the reverse effect. 

 

In view of the fact that I have had sleep apnea problems diagnosed twelve years ago, I decided to write and research the information about the sleep cycle to help others.  I have been sleeping well with the bipap machine for eight years.  The instrument ascertains that air pressure keeps the airways open preventing an oxygen interruption during the sleep cycle.  This report is a collection of different articles found on the internet.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

JET LAG:

 

Most people can feel when they are "not in synch" with their internal clock.  Lots of different things can throw you off, like traveling across different time zones.  Jet lag is a temporary condition characterized by fatigue and often disorientation which results from the lack of synchronization between the internal circadian rhythm and the external day/night cycle.  This sort of mismatch between the body's internal clock and the external day/night cycle can be an outcome of modern, technological lifestyles

 

An altered sleep pattern manifested by a sleepy feeling during the day and insomnia during the night is the most common characteristic of jet lag, but it may also cause indigestion and an inability to concentrate.  Jet lag may last for 25 or more hours until the circadian rhythm is stabilized.

 

HISTORICAL BACKGROUND:

 

Daily rhythms in plants and animals have been noticed since early times.  As early as the fourth century BC, Alexander the Great's scribe Androsthenes noted that the leaves of certain trees opened during the day and closed at night.

 

In 1729, French astronomer Jean Jacques d'Ortous deMairan conducted the first known experiment on biological rhythms.  He noticed that his heliotrope plant's leaves opened during the day and folded at night.  When he put the plant in total darkness the rhythm continued; it was not disrupted by the absence of daylight as an environmental cue.  Although he was interested in this botanical phenomenon, deMairan pursued astronomy instead.

 

Two centuries before modern gardeners noticed that their day lilies closed at night, the famous taxonomist Carolus Linnaeus discovered that the petals of many flower species opened and closed at regular times.  He even created a garden with flowers which opened at various times so that he could tell the time of day by looking in his garden.

 

Nothing exciting happened for many years... until the early 1900's, when Karl von Frisch observed that bees visited flowers only at specific times.  He and Ingeborg Beling trained bees to visit a nectar feeding station between 4 and 6 pm.  The bees did not visit at other times, and they still visited even when the nectar was removed.  When outside cues such as light were removed in laboratory trials, the bees still fed at prescribed times.  Although von Frisch did not know it, the bees were operating on an internal clock.

 

It wasn't until the 1950's that Gustav Kramer and Klaus Hoffmann proved the existence of a biological clock.  With an ingenious apparatus, Kramer demonstrated that starlings used the sun as a compass to migrate even though the sun itself moves throughout the day.  That is, the bird's internal clock reorients it in the direction of the moving sun. Hoffmann showed that the clock persisted in dim light and thus is endogenous to the animal.  He showed that the animal's clock was synchronized to local time by the influence of the local environment.

 

In the 1950's, Colin Pittendrigh demonstrated that circadian clocks are temperature compensated (have nearly the same period even when the temperature changes).  Most metabolic activities increase when body temperature increases, but the period of the biological clock does not.  Pittendrigh placed Drosophila pseudoobscura cultures at different temperatures and recorded the time of eclosion.  Even in constant darkness, the flies emerged on schedule regardless of the temperature.  Temperature changes thus did not affect the period of the clock [1].

 

THE HUMAN CLOCK:

 

Since the early experiments, chronobiology (the study of biological rhythms) has become established as an interdisciplinary field within biology.  Most chronobiologists study circadian rhythms, endogenous cycles of behavior or biological activity with a period of about 24 hours.  In the example, the human sleep-wake cycle has a period of 1 day, or goes through 1 complete cycle in a day.  Circadian rhythms, like the sleep-wake cycles discussed later, are generated by an internal clock that is synchronized to light-dark cycles in the environment and other daily cues.  Circadian rhythms are frequently plotted on an actogram.  An entraining agent (for example, exposure to bright light) can cause a phase shift (dotted line in the figure) whereby the activity is started earlier or later in the day.

 

Like a watch, the circadian clock must be synchronized to local time.  For example, animals kept in total darkness will show a free running rhythm that is independent of the local time.  A circadian clock is most useful, however, when it is set to local time; the animal must be in sync with its prey, pollinators, and other members of its social group in order to survive.  In mammals, the light-dark cycle is a major synchronizer or entraining agent for circadian rhythms [2].

 

WHERE IS THE CLOCK?

 

The clock in humans is located in the suprachiasmatic nucleus (SCN); a distinct group of cells found within the hypothalamus.  The SCN is only one part of the mechanism by which the "time" is kept.  There are light receptors found in the retina which have a pathway, called the retinohypothalamic tract, leading to the SCN.  The pineal gland is a pea-like structure found behind the hypothalamus in humans.  The pineal gland receives information indirectly from the SCN[3].  It appears that the SCN takes the information on day length from the retina, interprets it, and passes it on to the pineal gland, which secretes the hormone melatonin in response to this message.  Nighttime causes melatonin secretion to rise, while daylight inhibits it[3].  Even when light cues are absent, melatonin is still released in a cyclic manner; yet if the SCN is destroyed, circadian rhythms disappear entirely[4].

 

The role of melatonin in humans is not clearly understood and is currently being investigated, but it is thought to play a role in photoperiodism in seasonally breeding mammals.  The SCN is known to have hormone receptors for melatonin, so there may be a loop from the pineal back to the SCN.  Researchers now use melatonin levels as an accurate marker of the circadian rhythm in humans.

 

INFLUENCE ON BODY SYSTEMS:

 

The SCN also plays a role in the circadian system by triggering a neuroendocrine response in the hypothalamus, which then acts on the pituitary.  This last pathway profoundly influences other parts of the body, including the endocrine, immune, cardiovascular, and urinary systems.  Rhythms in most of these systems have a simple waveform similar to that of body temperature, which is highest in the early evening and lowest right before waking in the morning.  A plot of urine volume, for example, shows a cyclic pattern very similar to that of the body's temperature [4].

 

Some of the hormones thought to be influenced by the circadian system are growth hormone, prolactin, thyrotropin, and testosterone.  One endocrine event clearly under the influence of the clock is the release of the hormone ACTH.  The SCN triggers the hypothalamus, which activates the anterior pituitary to release ACTH, causing the adrenal glands to release cortisol and aldosterone.  A plot of cortisol concentration in blood plasma shows a characteristic peak in the very early morning (around 6 AM) with a trough right before bedtime.  Similarly, the aldosterone level is constantly high throughout the night and low throughout the day[4].

 

Activity of the immune system, as represented by the number of lymphocytes, also seems to peak in the late evening and is lowest a few hours after the cortisol peak in the morning[5].

 

The timing of the endocrine and immune systems are clearly intermeshed.  It is known that aldosteone and cortisol suppress the immune system, while melatonin appears to enhance it.

 

CIRCADIAN RYTHMS:

 

As living species evolved, their activities aligned to the day-night cycle caused by the Earth's rotation.  Initially, humans also responded to daily light-dark transitions, which served them well in hunter and agricultural societies.

 

Like other organisms, they developed real clocks to time biological processes.  These biological clocks measure events that occur once per day in what are known as "Circadian" rhythms (from 'circa'-about, 'dies'-a day).

 

Even in the absence of environmental time cues, such rhythms maintain a period close to 24 hours.  The circadian clock appears to regulate various aspects of metabolism, physiology and behavior, in humans as well as in other organisms.

 

By studying circadian rhythm anomalies in genetically mutated flies and other organisms, the mechanism has been identified to some extent.

 

Molecular and genetic studies indicate that a negative feedback loop is in order for the group to survive.  In mammantrols the transcription is the 24-hour clock genes.  While circadian rhythms appear to be similar in all species, it is unclear if this reflects a common mechanism passed along from a common evolutionary ancestor, or whether an outwardly similar process has arisen multiple times in evolution due to the stimulus of nature.

 

Evidence of circadian rhythms surrounds us.  In plants, the daily opening and closing of petals as well as leaf movements are the most visible, but circadian rhythms also control the discharge of floral fragrances and other metabolic activities associated with photosynthesis.

 

The circadian clock also influences seasonal cycles that depend on day-length, such as the regulation of flowering.  This photoperiodic system is quite likely dependent on the circadian clock's measurement of the duration of the day or night, thus monitoring the length and passage of seasons.

 

YOU'VE GOT RHYTHM:

 

A decade ago, the University of Florida Health and Human Performance newsletter printed a letter in which someone asked if jogging in the morning was risky because there is more of a chance of dying from a heart attack at that time of day.  The answer indicated that there was evidence that heart attacks are more likely to happen in the morning than at other times of day because of our circadian rhythms, our internal "body clock."

 

The origins of your body clock in all likelihood date back to the first living organisms.  As each species evolved, it developed a circadian rhythm that helped it survive within a specific environment.  For some this meant flying south in the winter.  For others hibernating.

 

For humans, it meant waking at dawn, hunting, working, eating, then retiring at dusk.  Our endocrine systems developed to release hormonal secretions that would help us obtain maximum results under those circumstances.  But, my how things have changed. Today some people go to work at midnight and sleep during the day.  Others awake at dawn and work well beyond dark.

 

As a society, we are ignoring the survival mechanism of our body, our own biological rhythms which regulate heart rate, oxygen consumption, cardio-pulmonary function, and hormone secretions.

 

Changes in the blood levels of the so-called circadian hormones (melatonin, cortisol and thyrotropin) as well as levels of growth hormone and glucose is used to measure our biological rhythms and how various activities affect these levels.  The release of melatonin is considered a key marker in an individual's body clock.  You might think of it as the starting point of the day, a moment after midnight, but the actual time it occurs in an individual varies.

 

The rhythms in a person's life are:

 

Ultradian Rhythms - These are shorter cycles, approximately 90 minutes in length.

 

Circadian Rhythms - Comprised of about 16 ultradian rhythms, the circadian rhythm regulates the sleep and awake cycles.

 

Circaseptan Rhythms – Seven-day cycles that appear to have been observed as far back in time as the origins of God creating the world…"on the seventh day he rested."

 

Infradian (Lunar) Rhythms - Most evident in a woman's menstrual cycle, this nearly month-long cycle has also been associated with the cycle of the moon.

 

Circannual Rhythms - Summer, fall, winter, spring demonstrate this cycle externally and it may explain why bears hibernate, birds migrate and some humans suffer seasonal mood changes.

 

Seven-year Rhythms - When one looks at childhood, puberty, adulthood, middle age and retirement, they are close to multiples of 7 years (7, 14, 21, 42, January).

 

Among the latest findings regarding this various rhythms are evidence that the body processes medicine better during certain periods of the day.  Pulse rate and blood pressure vary throughout the day.  Exercise is more beneficial during certain hours.  What you eat may be metabolized quickly at one time of day, but not another.  When you are dieting you should be more aware of your body clock.  Hormone levels vary throughout the day and month and years affecting sexuality.  Jet lag is caused by a disruption of our body clock.

 

Heart attacks and ischemic strokes caused by reduced blood flow to the brain have also been linked to circadian rhythms with morning the highest risk time and circannual peaks in March and September.

 

Ilaria Casetta, MD, from the University of Ferrara, Ferrara, Italy, and colleagues studied 1656 patients who were hospitalized with ischemic stroke, found that the strokes were more frequent during the first 2 hours after waking than at other times during the day. This confirmed earlier research findings that the peak onset of stroke symptoms occurred in the morning, with a second peak in the evening.  The morning increase is most likely in people 45 to 85.

 

The phenomenon may be associated with the body clock's morning rise in blood pressure and increase in the blood's clotting ability, according to the article Patient Demographic and Clinical Features and Circadian Variation in Onset of Ischemic Stroke in the January issue of the Archives of Neurology (2002;59:48-53).  The authors note that this circadian pattern is similar to that of a heart attack, sudden cardiac death, and other vascular events. They speculate that an underlying pathophysiological mechanism may be common.  This study was supported by a grant from the Italian Ministry of the University and Scientific and Technological Research, Rome.

 

Another pioneering study of a family of people who awake up to 3 ˝ hours before they want to, suggests that there is a gene that controls circadian rhythms and the regulation of sleep.  This gene may vary in families and thus each body clock is inherited from our parents.  The study, which was reported in the Archives of Neurology (2001;58:1089-1094) was conducted by The Departments of Neurobiology and Physiology, and Molecular Pharmacology and Biological Chemistry, the Transportation Center, the Center for Circadian Biology and Medicine, and the Howard Hughes Medical Institute, Northwestern University; and the Department of Neurology, Northwestern University Medical School, Chicago, Ill.

 

BIOLOGIC RHYTHM DISRUPTION:

 

Ultradian Rhythms and Insomnia:

Embedded within the daily circadian rhythm are approximately sixteen 90-minute Ultradian rhythms.  Often these go unnoticed due to the pace and schedules in our lives. One measure of these activities is the Rapid Eye Movements (REM) and Non-REM periods of sleep.  When these get disrupted people experience insomnia and the resulting fatigue.

 

Circadian Rhythms, Metabolism and Eating Disorders:

Circadian rhythms are evident everywhere in nature.  In plants, petals open and close as the sun rises and sets and metabolic activities associated with photosynthesis control the discharge of floral fragrances and other plant activities.  Animals in the wild follow a daily cycle of awakening, hunting, eating and resting.  Humans on the other hand, tend to ignore their circadian rhythms, which control all hormonal activity and metabolism.  As a result they feel sluggish when they should be awake and awake when they should be sleeping.  And their eating habits have made the society as a whole overweight.  They feast on a diet of junk food, diet pills, antidepressants and painkillers to get through the day.

 

SOLUTIONS TO BIOLOGIC DISRUPTION:

 

1.  Light therapy may also be helpful in resetting a person's body clock when sleep cycles have been disrupted by too much work, partying or jet lag.

 

2.  Some researchers believe that a combination of light therapy, sauna therapy, meditation and exercise with restorative recovery are essential for resetting the body clock.

 

3.  Sauna Therapy:  Some also substitute Sauna Therapy for a cardiovascular workout since it can burn calories without muscular and joint stress.  In mamleans clogged pores help to relieve acne, eczema, psoriasis and burns.

 

4.  A cycle of sauna activity can help mimic the temperature cycles that are part of the body's natural rhythms.  Typically the highest temperature in the human body occurs around 4 p.m. and the lowest at 4 a.m.

 

5.  Meditation:  One approach to restoring circadian rhythms is to use the process of meditation to help eliminate the stress that has disrupted the body clock.  Many ancient civilizations have long recognized that meditation is as effective in restoring energy as a nap can be.

 

6.  When animals are observed in the wild they tend to exercise in short bursts of activity followed by a recovery cycle.  This is similar to the wave of activity seen in heart waves and may be the most natural approach to exercise that maximizes the benefits of innate circadian rhythms.

 

CIRCADIAN RYTHM AND INTERACTION WITH JET LAG OR TIME ZONES:

 

Circaseptan Rhythms, Medical Implications and Sexual Dysfunction:

The ebb and flow of countless hormones that control life's processes are intertwined with human biological rhythms.  While these secretions occur within ultradian and circadian time frames, they also appear to fall within a circaseptan rhythm (the seven-day cycle).

 

Many physicians believe that transplant patients tend to have more rejection episodes seven, fourteen, and twenty-one days after surgery.  In western civilizations and urbanized cultures, heart attacks are most frequent on Monday mornings, possibly associated with the shift from leisure to work.

 

Today's hectic pace also takes its toll on sexual activity.  While some studies indicate that the typical married couple is intimate 2-3 times a week and usually on Tuesday nights, others studies report a rising number of couples that report no contact for weeks on end. It may be that their biological rhythms are so out of sync that "the chemistry" between them has disappeared.

 

Infradian (Lunar) Rhythms and Hormones:

The 28-30 day Infradian rhythm has long been recognized as a phenomenon that not only affects tides, but also hormones.  The most obvious is the menstrual cycle in women with fluctuating levels of estrogen and progesterone.  There are some studies that suggest that the timing of breast cancer surgery within the hormonal cycle of pre-menopausal women can have a significant impact on the course of the disease.

 

Circannual Rhythms and Mood Disorders:

Seasons are part of the circannual rhythm seen in the blooming of flowers in the spring, the changing of leaves in the fall, the rise of temperatures in the summer and the winter's cold and snow.  These seasonal cycles depend on day-length, and the shortened days seem to have an effect on people who suffer from a mood disorder known as Seasonal Affective Disorder (SAD).

 

It is believed that deep within the brain, two clusters of cells get information from photoreceptors in the retina, which transmit signals about light and dark through the optic nerves to the hypothalamus.  In response, the pineal gland regulates the secretion of melatonin, which may result in the production of serotonin.  In mamlved, several central psychological processes occur including pain perception, temperature and blood-pressure regulation, and several neuropsychological functions such as appetite, memory and mood.  The photoreceptors in some people require more light to stimulate the hypothalamus than others, without which they will suffer SAD.

 

Out beyond the yearly circannual biological rhythm there appears to be seven-year cycles that have been noted by the seven-year itch in marriage, but interestingly the typical homeowner also holds onto their first house for approximately the same period of time. The itch may have less to do with lust than with a biological rhythm related to wanderlust.

 

In animals, the circadian clock controls all hormonal activity-which means that if your circadian clock is off you will feel the effects in your metabolism, sexual appetite, sleep patterns and tolerance to stress.  Problems with your circadian clock can cause symptoms in the following systems of your body:

 

THE CLOCK IN OTHER ORGANISMS:

 

Non Human Animals:

 

Research in the area of biological clocks did not begin with humans.  Early research was done on a variety of animals including rats, hamsters, sparrows, lizards, marine snails, and fruit flies.  The choice of an animal depended on many considerations--size, rate of reproduction, expense to maintain, and availability--as well as behavioral issues, such as whether it entrained easily and what environmental factors seemed to affect its behavior.

 

House sparrows were one of the first animals in which the biological clock was anatomically localized [23].  The sparrow's clock is believed to consist of the suprachiasmatic nucleus (SCN), as in humans, and the pineal gland.  In this circadian system, photoreceptors are found in the retina as well as in the pineal gland and in the deep brain.  Investigators are clarifying the relationship between the sparrow's two clocks and its photoreceptors [24].

 

Although sparrows are similar to humans, how do they compare with other vertebrates such as reptiles?  Reptiles also have photoreceptors in the eye, the pineal and in the deep brain.  Most reptiles and some amphibians, however, also have photorecptors in the parietal eye, an unseeing third eye located on the top of the skull with a well-defined retina, lens, and cornea that is known to play a role in thermoregulation and photoperiodism in some species [25].  In reptiles and other lower vertebrates, the pineal gland synthesizes information from the photoreceptors and in turn secretes melatonin in a rhythm which synchronizes other parts of the clock system.  The role of the SCN in lower vertebrates is unclear, but it is thought to contain another part of the clock.

 

One of the most intensively studied invertebrates is Drosophila, the familiar fruit fly.  This insect reproduces quickly, is economical to raise, requires very little space, and has an extensively mapped genome.  Locomotor activity is difficult to observe in individual flies, but the eclosion rhythm, which is under clock control, can be observed in large numbers of flies [23].  Mutant strains of flies are presently being used to discover more about the molecular basis of the clock.

 

The biological clock has been found in the optic lobe in other invertebrates, such as cockroaches, crickets and silkmoths [23].  Mollusks seem to have a clock only in the eyes.  Two types of mollusks, a marine snail (Bulla gouldiana),  pen and ink of Bulla by Donna Bennet,and the sea hare (Aplysia californica), have large neurons that can be individually studied [see review by 23].  Furthermore, isolated retinal neurons can even be placed in vitro, and still exhibit circadian rhythms in their electrical behavior.

 

The most widely studied mammals are rats, mice, and hamsters. All of these rodents have clearly defined circadian rhythms measured as the onset of their daily locomotor activity. Hamsters, for example, are nocturnal and will become active at as a group in order to survive.  In mamcertain time every night, this rhythm can be quantified by recording their activity on a running wheel which is connected to a computer that converts the raw data into an actogram.

 

The SCN was first identified as a circadian clock in rats.  When Richter lesioned the rat SCN, the animals' locomotor activity became arrhythmic.  This experiment was soon followed by a series of investigations in other rodents and mammals on how SCN lesions can affect circadian rhythms [23].

 

Late in the 1980's a spontaneous mutation, later named the tau mutant, was discovered in a colony of golden hamsters.  The period of the mutant's rhythm was noticeably shorter than the 24-hour norm: heterozygous tau mutants had approximately 22-hour periods, and homozygous ones had a 20-hour cycle.  When normal hamsters who had been made arrhythmic by a SCN lesion were given a transplanted tau SCN, they resumed a circadian rhythmicity that matched that of the donor.  This experiment was also repeated in reverse, with the tau hamster showing a normal hamster's 24-hour rhythm after receiving a normal hamster's SCN.  These experiments provided the final proof that the SCN is the site of a clock in mammals [26].

 

July 26, 2002:

Center researcher Mike Menaker observes the behavior of a tau mutant.  A similar mutant gene (clock) that causes a longer than normal period of running wheel behavior was recently found in mice.  This discovery is exciting because mice have a well-mapped genome.  Investigators hope to use hamster and mouse mutants to identify the clock's internal mechanisms and determine at the genetic level what makes it "tick."

 

PLANTS:

 

Charles Darwin is probably most famous for his ideas on evolution through natural selection.  In his later years, he became fascinated with plant movements while trying to find an evolutionary relationship among them.  He did hundreds of experiments to keep track of the movements of the different varieties of plant leaves.  After experimenting, Darwin concluded that the plants were moving their leaves so as to expose the smallest possible leaf surface to the night temperatures.  His book, The Power of Movements in Plants,details the years of work.  In the years following, scientists would debate whether the rhythm arose from forces external to the plant or was endogenous.

 

In 1920, a landmark paper was written by W.W. Garner and H.A. Allard in which they showed that tobacco plants would flower only if exposed to a certain number of hours of light.  The term "photoperiodism" was used to designate the response of the organisms to relative length of day and night.  Garner and Allard showed that plants could tell time! The ability to sense day length is an important ability for plants so that they grow, reproduce and develop during favorable time of the year.  The changing times of dawn and dusk contain seasonal information as well as time of day information so that the organisms have, in effect, an internal clock and calendar[23].

 

In plants, a photoperiodic clock not only controls flowering, but also induction and termination of dormancy in buds and bulbs, seed germination, and daily rhythms such as leaf movements, petal movements, and nectar secretion.

 

In more recent experiments with plants, Steve Kay et al has developed an interesting technique to measure rhythms in Arabodopsis thaliana.  The researchers chose this plant for many reasons including its small size, short life cycle, and number of chromosomes (n=5).  As a way to measure rhythmic gene expression in vivo in these plants, they transplanted firefly luciferase gene (which is responsible for the insect's glow) into the plants.  The plants exhibited rhythms in bioluminescense when their CAB genes (CAB is a light harvesting protein) were being "turned on" rhythmically. In other words, the luciferase gene was used as an intra-cellular marker to detect the plants' own gene activity  rhythms[27].  In other experiments, they isolated and cloned a photoreceptor gene from rice.  Multiple copies of this ps group are needed in order to survive.

 

In mamhytochrome, genes were transplanted to tobacco with the result that the tobacco plants became hypersensitive to light because of the higher than normal number of photoreceptors[28].  Scientists are attempting to do a similar experiment with rice in the hope that they will be able to grow rice under low-light conditions and produce more crops per year.

 

 

 

The Restless Legs Syndrome:

By: Richard P. Allen, Ph.D.

 

Outline

General Description

Daily Cycle of Symptoms and the Relation to Sleep

Symptoms in Relation to Activity

Diagnosis

Medical Conditions Associated with RLS

Treatment Approaches

Support Group and the RLS Foundation

Recommended Articles in Medical Journals

General Description

 

The Restless Legs Syndrome (RLS) is a fairly common sleep-related neurological disorder affecting, in some form, about five percent of the adult population.  It is one of the older recognized sleep disturbances, first described over 300 years ago. Frequently seen with greater frequency in particular families, it is often reported to be a genetic disorder, although this has yet to be confirmed. RLS causes disturbing sensations and it leads to a need for excessive amounts of movement.  When the strange sensations, or sensory symptoms, are clearly present, the diagnosis is fairly easy. The RLS patients describe peculiar, very disturbing sensations localized in the limbs, usually the legs.

 

The abnormal sensations are often hard for patients to describe since they have no reference in normal life experience.  They are commonly described as: "Pepsi in the veins," "worms under the skin," "crawly feelings in the legs," "electricity running through the legs," etc.  The sensations are painful for about 30% of the RLS patients, but they are almost always compelling. 

 

There is a wide variation in severity.  Some patients experience the sensations only occasionally at night, while others have intense feelings every day leaving them almost frantic to find relief even when they are not associated with pain.  It is the compulsion to remove the feelings that leads to excessive movements, since the best form of relief is walking or any large body movement. 

 

Sometimes the compulsion to remove the sensation becomes the dominant feeling, and the patient reports primarily this irresistible urge to move the legs. The feelings generally wax and wane occurring about once every 5 to 20 seconds.  When the patient gets up and walks around, these feelings generally go away, but they quickly return when the patient stops walking.  Fortunately some reasonable treatments are now available for RLS.

 

Daily Cycle of Symptoms and the Relation to Sleep:

 

The symptoms have a marked circadian (daily) pattern becoming much worse in the evening to early morning and often completely abating in the later morning.  When severe, the symptoms will start in the afternoon or even shortly before noon.  In some very extreme cases the symptoms occur 24 hours a day, but even then are generally less severe in the morning.  In almost all cases, however, there is a relative sparing of the early morning hours from about 7-10 AM, when there are virtually no symptoms. Patients nonetheless live in dread of the next bedtime when they will again be struggling with these feelings.  Nighttime brings not rest but suffering.

 

This daily cycle expresses itself in the nature of the sleep disturbances with RLS.  The symptoms make it very hard to fall asleep.  To make matters worse, once asleep, repeated periodic leg movements usually occur throughout the first several hours of sleep.  Even in sleep, the legs cannot be still. These movements occur about once ever 5-to-40 seconds. Many movements cause brief arousal, disrupting sleep without awakening the patient. Some actually cause awakening, with return of the wake-time urge to get up and walk. The sleep itself is of poor quality that does not fully restore alertness for the next day. When mild, RLS disturbs mainly sleep onset and the first hour or two of sleep, with better, even normal, sleep occurring later in the morning.  When severe, RLS disturbs sleep most of the night with only some partial relief in the very last part of the morning.

 

The more severe RLS patient may be getting no more than 4 hours of sleep a night.  The rest of the night the patient is up wandering around with intermittent and only partially effective attempts to sleep.  The symptoms sometimes appear to come on in bouts with temporary relief after some walking around.  The patient can then get some restless sleep before the next bout of the distressing sensations.  The bouts finally may abate two to four hours before the time to wake up.  This profound sleep deprivation increases sleepiness the next afternoon and evening and appears to make the disturbing sensations worse.

 

Symptoms in Relation to Activity:

Another of the peculiar diagnostic features of this disorder is the onset during rest. Symptoms start when the patient has been sitting or reclining for a while. Symptoms are also exacerbated by any sedentary or resting activity.  The most common activities reported to bring out symptoms include: sitting in a movie or lecture; riding in a plane or car; and, of course, lying down to rest or sleep.  The longer the period of inactivity the more pronounced the symptoms may become.

 

Conversely, motor activity, particularly walking, provides almost immediate relief of symptoms that lasts as long as the walking continues.  It is probably not the movement itself that produces the relief but rather the alerting effects of the movement.  Other activities reported to produce relief are those that produce profound alertness such as: inflicting mild discomfort by hard rubbing or very hot baths, engaging in active social interaction such as in an emotional argument and becoming totally engrossed in needle arts or a computer game.  It is interesting that standing still does not produce much relief, but standing on one leg is generally as effective as walking.  Standing on one leg requires alert focused attention.

 

The overall picture is of a disorder gated by time of day but triggered by activities that lead to sleepiness and relieved by doing something to become fully awake.  Patients tend to develop an RLS life style characterized by lots of motor activity and little 'down time' particularly after noontime.

 

Diagnosis:

A recently formed International Restless Legs Study group developed the currently accepted diagnostic criteria for RLS.  ALL of the following four criteria are necessary and sufficient for the diagnosis:

 

1. Urges to move the legs usually associated with abnormal sensations (paresthesias).

2. Motor restlessness, including one or both of two types: a) voluntary movements to reduce symptoms, and b) smooth, short (0.5-10 second) bursts of involuntary, usually periodic, limb movements occurring mostly when the patient is reclining.

3. Onset or exacerbation of symptoms by rest and marked relief by activity, particularly walking.

4. A pronounced circadian (daily) pattern with symptoms significantly greater in the evening during the sleep time (maximal between 10 PM and 2 AM) and much less later in the morning.

 

Medical Conditions Associated with RLS:

RLS appears to occur secondary to the development of four other recognized medical conditions: iron deficiency, later stage kidney disease, pregnancy and peripheral neuropathy (abnormal sensations in the hands or feet) and radiculopathy (pain which occurs over the distribution of spinal nerves).  Cases where RLS occurs after the development of one of these conditions are usually considered to be "secondary RLS," but it should be noted that not all patients with these conditions develop RLS.  In other words, RLS occurs for a large number of patients after one of these conditions develops, but the conditions themselves do not suffice to produce RLS.  Moreover, these conditions also exacerbate the severity of RLS for patients who have RLS before developing the condition.

 

Treatment Approaches:

Evaluation of medication treatment for RLS is complicated by the marked daily variation observed in the severity of these symptoms and the different way each person experiences the symptoms.  In general, treatments must be tailored to the severity of the disorder.  The following approaches should be considered.

 

Iron Treatment:

Our work at Johns Hopkins has clearly identified a problem with low iron levels for RLS patients.  The iron measurement that we are most interested in is called ferritin.  The level of ferritin in the blood is critical in RLS and as a conservative rule should be kept above 50 mcg/l.  When ferritin is low an iron supplement should be given.  Tablets of 325 mg of iron sulfate can be obtained from the pharmacist.  The dose should be adjusted in consultation with a doctor.  Commonly, one iron tablet is taken 3 times a day with a 500 mg tab of vitamin C to help the body absorb the iron.  Iron has the usual side effect of constipation that should be conservatively managed.

 

Behavioral Management:

RLS symptoms can be reduced somewhat by the behavioral techniques described above: walking, hard rubbing of the legs, very hot baths, arguments, and engrossing needle work or video games.  Patients commonly report that if they become active when they first feel the onset of the symptoms, they can prevent the development of the symptoms and later return to being inactive.  If, however, they attempt to delay activity as long as possible, the symptoms become more pronounced and then reoccur whenever they return to inactivity.

 

RLS patients also often develop very poor sleeping habits.  As a result of many nights with very disturbed sleep onset, some patients develop a physiologic insomnia complicating their RLS problems.  Then, when the leg movements are reasonably well managed with medications, they will have a persisting physiologic insomnia that may require behavioral treatment.

 

Please see our article, "Insomnia," for a full discussion of the treatment of physiologic insomnia.

 

Medications to Avoid:

Before starting medications to treat RLS, it is often useful to ensure that the patient is not taking any medication that exacerbates RLS.  Patients generally report that all of the drugs in the "dopamine antagonist" family (drugs which decrease the effect of the chemical, dopamine, in the brain) exacerbate their RLS.  Thus, certain antipsychotic drugs and drugs for nausea should generally be avoided or used only in lower doses.

 

Most anti-depressants exacerbate RLS. The one exception is buproprion (Wellbutrin) which as a dopamine agonist (a drug which increases the effect of dopamine in the brain) could possible have some mild benefit for RLS.  Some patients also report that the sleep benefit of trazadone (Desyrel) outweighs its exacerbation of RLS.

 

Medications:

For very mild RLS, one option is to use a hypnotic agent (sleeping pill) to promote sleep. The hypnotics that have been shown to work include Klonopin (clonazepam), Restoril (temazepam), and Halcion (triazolam).  Ambien (zolpidem) is likely to work, but has not been adequately studied.  Klonopin is the most studied of these medications, but it also is very long acting and likely to produce daytime sedation and should therefore be used with caution.

 

The most effective drugs for RLS are the drugs that affect the "dopaminergic" nervous system by increasing the amount or effect of the chemical dopamine in the brain.  One of these drugs, a combination of levodopa with carbidopa called Sinemet, remains the most effective drug for immediate relief of symptoms.  A very low dose (1/2 to 1 tab of Sinemet 25/100) can provide virtually complete relief from symptoms.  This medication is usually effective within 30 minutes and lasts about 3 hours.  It can be used during the day when RLS symptoms occur during sedentary activities and is particularly helpful for long plane rides or car trips.  For those patients who don't experience symptoms every night, it can be used on an "as needed" basis, on nights when a patient is experiencing symptoms. 

 

Unfortunately the medication does not last long enough to give relief for the full sleep period and is rarely adequate to manage any other then mild RLS symptoms.  The major problem with treating RLS with Sinemet is that, over time, the symptoms of RLS become more severe.  This is referred to as "augmentation."  Therefore, what was once a mild-to-moderate case now gradually becomes a severe case.  symptoms that were once limited to the evening start occurring during the afternoon and even the morning.  To avoid this problem, it has been recommended that the use of this medication be limited to no more than 2 tabs of Sinemet 25/100 a day.

 

A number of other drugs which mimic the action of dopamine in the brain (dopamine agonists) are also considered excellent medications for treatment of all but the most mild RLS.  Pergolide (Permax) is well studied and commonly used in divided doses in the evening, starting at a very low dose (1/2 tab 0.05 mg at each dose time) with very gradual dose increases until symptoms are relieved or significant adverse effects occur. Augmentation occurs for about 15% of the treated patients, but is usually mild.  It may again be dose related, so total daily doses above 0.75 mg should be used with caution. Two newer dopamine agonists, Mirapex and Requip, seem likely to work with RLS, but at this time they have not been as well evaluated as pergolide.  The major side effects of this type of medications are nausea, vomiting, stuffy nose and dizziness on standing.

 

The second line of medications that help RLS include drugs that are used to treat pain, known as the opiates.  Oxazepam has been found effective in clinical studies, but it is likely that all of the drugs in the opiate family will have some benefit.  They are often used in combination with dopamine agonists.  They are also used in cases where dopamine agonists fail, either because of adverse effects or augmentation.

 

For special cases, particularly those involving RLS symptoms that are reported to be painful, the use of the anticonvulsant Neurontin (gabapentin) has also been used successfully.

 

There are a number of other medications that have been used with mixed success, but none have the demonstrated effectiveness of those mentioned above.

 

Given the wide range of medication alternatives, it is recommended that if a patient does not experience improvement with one of the standard single drug therapies (with either a hypnotic or a dopaminergic medication), consultation be obtained from an expert in RLS. Accredited sleep disorder centers are required to have expertise in treatment of this disorder and are likely to be the one good source of quality care for this condition.

 

Support Groups and the Restless Legs Syndrome Foundation:

The patients with RLS have formed the RLS foundation, which is very active in providing education about RLS and supportive services for patients.  They publish a newsletter called Nightwalkers, available by subscription.  The RLS Foundation can be contacted at 4410 19th St. NW, Rochester, MN 55901-6624.

 

Light Therapy:

For people with Seasonal Affective Disorders (SAD) many people are experimenting with Light Therapy.  This approach helps stimulate the brain's photoreceptors, which for some people, seem to crave the light stimulation that is at its maximum in the summer. Light therapy may also be helpful in resetting a person's body clock when sleep cycles have been disrupted by too much work, partying or jet lag.  Some researchers believe that a combination of light therapy, sauna therapy, meditation and exercise with restorative recovery are essential for resetting the body clock.

 

Aging alters sleep and hormone levels sooner than expected.

 

Contact:  John Easton , 773-702-6241

Embargoed Until:  3 p.m. CT, Tuesday, August 15, 2000

 

Researchers from the University of Chicago report in the August 16 issue of JAMA that age-related deterioration of sleep quality occurs in at least two stages, and that, for men, the first stage occurs sooner than expected -- between the ages of 25 and 45.  They also found that changes in sleep were mirrored by changes in hormone secretion.

 

This was the first study to examine sleep quality and hormones influenced by sleep throughout adulthood, rather than comparing the sleep patterns and hormones of young versus old.  The researchers collected data from sleep studies conducted between 1985 and 1999 on 149 healthy men aged 16 to 83.

 

"Our study maps out the chronology of age-related changes in sleep duration and quality and suggests that altered levels of certain hormones may be a consequence of sleep decay," said Eve Van Cauter, Ph.D., professor of medicine at the University of Chicago and director of the study.  "These changes in sleep quality provide an early biological marker of aging in men."

 

The first stage of deterioration of sleep due to aging occurs between young adulthood (ages 16 to 25) and mid-life (35-50).  Although total sleep remained constant as young adults moved into mid-life, the proportion of slow wave or deep sleep decreased from nearly 20 percent of a normal night's sleep for those under 25 to less than five percent for those over 35.  Growth hormone secretion, which occurs primarily during deep sleep, also declined by about 75 percent.

 

By the age of 45, note the authors, most men have almost entirely lost the ability to generate significant amounts of deep sleep.  This study suggests that, as a consequence, most middle-aged men have very low levels of growth hormone.

 

Growth hormone deficiency has been studied extensively in the elderly, where it is associated with increased obesity, loss of muscle mass and reduced exercise capacity.

This study suggests that clinical trials of growth-hormone replacement therapy, which have previously been conducted in older men and women, might better target individuals in early mid-life.  People over 65, who have already lived without the hormone for decades, may be less likely to respond and more likely to suffer adverse side effects.

 

"We begin estrogen replacement as soon as women enter menopause, not 20 years later," said Van Cauter.  "If men go through 'somatopause' -- a loss of growth hormone -- between 25 and 45, why should we wait another 20 years to initiate treatment?

 

Another option is an indirect form of hormonal therapy, supported by recent studies in the Van Cauter lab and elsewhere, that involves using investigational drugs to stimulate increases in deep sleep.  Increasing deep sleep triggers a proportional increase in growth hormone secretion.

 

The second stage of deterioration of sleep due to aging occurs after age 50, during the transition from mid-life to late life.  It includes decreased total sleep -- which declines by about 27 minutes per decade – more frequent and longer night-time awakenings, and a significant reduction in REM or dream sleep, to about 50 percent of young-adult levels. The loss of REM sleep appears to be associated with elevated evening levels of the stress-related hormone cortisol.

 

Cortisol is a 'fight or flight' hormone that heightens attention and alertness.  Levels normally peak in the morning and decline during the day to very low levels in the evening, giving the system time to recover.  Subjects with decreased REM sleep, however, had "an impaired ability to achieve evening quiescence," note the authors.

 

This lack of hormonal "down time," a recovery period for the stress-response system, has been linked to memory deficits and insulin resistance, a risk factor for diabetes.  Elevated evening cortisol levels could also cause additional sleep loss.  "Our data support the concept that decreased sleep quality contributes to the wear and tear resulting from overactivity of the stress-responsive systems," said Van Cauter.

 

By mapping out the effects of aging on sleep quality and hormone production, this study suggests new ways to intervene in the aging process.

 

"It is a tantalizing concept," said Van Cauter. "We are developing medications that can, in part, restore the capacity for deep sleep. If we could slow down the age-related changes in sleep quality, would that delay some of the many hormonal consequences of growing older?"

 

Additional authors of the paper were Rachel Leproult, M.S., and Laurence Plat, M.D., both from the Van Cauter laboratory.  The data were collected from a series of sleep studies; 109 subjects were studied at the University of Chicago and 40 were studied at the University of Pittsburgh, UCLA, or Pennsylvania State.

 

This research was supported by grants from the National Institute on Aging, the National Institute of Diabetes and Digestive and Kidney Diseases, and by the Mind-Body Network of the MacArthur Foundation.

 

Summary: University of Chicago researchers report that age-related deterioration of sleep quality occurs in at least two stages.  For men, the first stage occurs between the ages of 25 and 45.  Changes in sleep are mirrored by changes in hormone secretion.  This finding suggests new approaches to hormone replacement for men.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

BIBLIOGRAPHY

 

 

Recommended Articles in Medical Journals:

 

Allen, RP, Earley, CJ (1996a). Augmentation of the Restless Legs Syndrome with Carbidopa/Levodopa. Sleep, 19: 205-213.

 

Kaplan, P., Allen, R., Buchholz, D., & Walters, J. (1993). A double-blind, placebo-controlled study of the treatment of periodic limb movements in sleep using Carbidopa/Levodopa and Propoxyphene. Sleep, 16: 717-723.

 

Earley, C., Yaffee, J., & Allen, R. (1999). Double-blind, placebo-controlled assessment of Pergolide treatment of the restless legs syndrome. Neurology.

 

Sun, E., Chen, C., Ho, G., Earley, C., & Allen, R. (1998). Iron and the Restless Legs Syndrome. Sleep 21:381-387.

 

Walters, A., Aldrich, M., Allen, R., Ancoli-Israel, S., & al., e. (1995). Towards a Better Definition of the Restless Legs Syndrome. Movement Disorders, 10: 634-642.

 

Several publications from the Sleep Apnea Association (APA) on Sleep Apnea and Sleep Disorders.